Abstract
ABSTRACT. The glutamate oxaloacetate transaminase (GOT) zymogram phenotypes of 54 wheat strains, each possessing a distinctive chromosomal constitution, were determined. Three of the several genetically independent GOT systems expressed were analyzed. The results obtained support the hypothesis that the active GOT isozymes of each system are dimers composed of the six possible combinations of subunits coded by triplicate structural genes. Sets of triplicate GOT structural genes were linked to the chromosome arms 3Aα, 3BL, and 3Dα, to 6Aα, 6BS, and 6Dα, and to 6Aβ, 6BL, and 6Dβ. Genes involved in the production of GOT isozymes were also located in the chromosomes of homoeologous group 7. Tissue specificity in the expression of the GOT systems was observed. The results of this study suggest that genetic regulation of the expression of the GOT isozymes occurs at the level of gene transcription and that the rate of transcription is the same for each copy present of each of the three possible members of the homoeologous sets of GOT structural genes.
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