Abstract
Objective To investigate the fibrosis-associated genes expressed in the tissue of extensive fibrotic kidney of patients with severe hydronephrosis.Methods Twelve extensive fibmtic kidneys in patients with severe hydronephrosis(hydrnephrosis group)were compared with 6 normal renal tissues (control group)by using real-time polymerase chain reaction(PCR)assay.All samples were collected from our hospital from Oct.2005 to Aug.2007.The results of real time PCR assay were processed with ΔΔCt method.When 2~(ΔΔc1) wag≤0.5 or≥2.the difference Was considered significant.Then the expression of left-right determination factor A(LEFTY-A)in both groups were detected by immunohistochemistry.Results A total of 49 differential genes expressed in the fibrotic renal tissues were screened out.of which,25 were up-regulated and 24 were down-regulated.The differential genes included members and their receptors of transforming growth factor(TGF)-β super family or bone morphogenetic protein(BMP) family,target molecular of TGF-β or BMP signal pathway and several relative regulators.And the gene of LEFTY-1.one of the most markedly-changed genes,in hydrnephrosis group Was 13.55-fold down-regulated as compared with control group.Immunohistochemistry analysis reveled that LEGTU-A protein in the sample of hydronephrosis group was significantly decreased as compared with that in control group(P＜0.05).Conclusion The LEFTY mRNA and protein expression Was decreased in human severely hydronephrotic renal tissue.indicating that LEFTY may play an important role in the inhibition of renal fibrosis resulting from long-term hydronephrosis. Key words: Hydronephrosis; Reflal fibrosis; Gene chip; Left-right determination factor
Published Version
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