Abstract
Polyunsaturated fatty acids (PUFA) are highly susceptible to oxidation and their levels can rapidly decrease under oxidative stress [1]. A concomitant increase in monounsaturated fatty acids such as oleic and palmtoleic acids is often observed probably as a result of the activation of Δ9-desaturase [1]. Analysis of fatty acid profiles is therefore of importance. Here we describe the analysis of plasma free fatty acids (FFA) only, because the measurement of fatty acids in neutral lipids, phospholipids, and total lipids has already been well documented. Plasma FFA can, furthermore, be produced by oxidatively damaged tissues because hydrolysis of phospholipids is stimulated by oxidative stress [2].
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