Abstract
Analysis of the flavonol glycoside patterns of 13 Epilobium species was carried out by thermospray liquid chromatography-mass spectrometry (TSP/LC-MS) and high performance liquid chromatography coupled with a photodiode array detector (LC-UV). Mass-data and UV spectra recorded online, before and after post-column addition of shift reagents, provided useful structural information on the different compounds present in crude extracts. Nineteen flavonol glycosides were identified by this means. In order to confirm the on-line assignments, 13 of the compounds were isolated and their structures were elucidated. They were all 3- O-glycosides of kaempferol, quercetin and myricetin.
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