Abstract
The determination of saponins in crude plant extracts by liquid chromatography—thermospray mass spectrometry is described. The method is shown to be suitable for the separation, identification and determination of saponins carrying up to three sugars. The mass spectra recorded on-line provide information on the molecular mass, the nature of the sugars and their sequence. Selected applications for saponins from the molluscicidal plant Tetrapleura tetraptera are described.
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