Analysis of Flavonoid in Medicinal Plant Extract Using Infrared Spectroscopy and Chemometrics.

Lestyo Wulandari,Nuri Nuri,Hilmia Lukman,Yuni Retnaningtyas

doi:10.1155/2016/4696803

Abstract

Infrared (IR) spectroscopy combined with chemometrics has been developed for simple analysis of flavonoid in the medicinal plant extract. Flavonoid was extracted from medicinal plant leaves by ultrasonication and maceration. IR spectra of selected medicinal plant extract were correlated with flavonoid content using chemometrics. The chemometric method used for calibration analysis was Partial Last Square (PLS) and the methods used for classification analysis were Linear Discriminant Analysis (LDA), Soft Independent Modelling of Class Analogies (SIMCA), and Support Vector Machines (SVM). In this study, the calibration of NIR model that showed best calibration with R 2 and RMSEC value was 0.9916499 and 2.1521897, respectively, while the accuracy of all classification models (LDA, SIMCA, and SVM) was 100%. R 2 and RMSEC of calibration of FTIR model were 0.8653689 and 8.8958149, respectively, while the accuracy of LDA, SIMCA, and SVM was 86.0%, 91.2%, and 77.3%, respectively. PLS and LDA of NIR models were further used to predict unknown flavonoid content in commercial samples. Using these models, the significance of flavonoid content that has been measured by NIR and UV-Vis spectrophotometry was evaluated with paired samples t-test. The flavonoid content that has been measured with both methods gave no significant difference.

Highlights

Indonesia shows an amazing diversity of plants species that have been associated with the human health from time immemorial
Samples used were leaves samples collected from Materia Medica Botanical Garden, Malang, Indonesia (Table 1)
The monochromator entrance slit was set on 500 pm, the amplifier was set on 200. the response time is smooth (1 ms), and light intensity was set on 14 volts

Summary

Introduction

Indonesia shows an amazing diversity of plants species that have been associated with the human health from time immemorial. Research of Indonesian medicinal plants using modern laboratory facilities has been started since 1970, but only about 200 plants were studied. We show a very small portion of the overall number of medicinal plant species that were reported [2]. The analysis of chemical constituents would help in determining various biological activities of plants. Studies have shown that many plants have chemical components and biological activities. The most important of these bioactive constituents of plant are alkaloids, flavonoids, terpenoids, steroids, tannins, and saponins [3]. Flavonoids have been reported to exert multiple biological effects, including antioxidant, free radical scavenging abilities, antiinflammatory, and anticarcinogen [5]

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Conclusion