Analysis of Fibrous Compounds Using a Pressurized and Non-pressurized Conditions

Edgard Gonçalves Malaguez,Gabriela Ceratti Hoch,Francisco Augusto Burkert Del Pino,Deise Dalazen Castagnara,Kelli Flores Garcêz ,Andressa Tellechea Rodrigues ,Ana Paula Schmidt

doi:10.5296/jas.v8i4.17591

Abstract

Neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents were evaluated in pressurized and unpressurized conditions using samples of roughage and concentrates. In summary, the samples were dried, processed in a knife mill, weighed in nonwoven bags (100g/m²), placed in a container and treated with neutral or acid detergents. Extractions of NDF and ADF content were carried out in a non-pressurized condition at temperature of 100°C for 60min and in pressurized condition using different temperatures of 100 and 110°C for 60min. Results of the different temperatures using the pressurized procedure were compared to those obtained with the pressurized through the linear regression analysis. The method with the temperature 110°C for 60 min had a high level of agreement. Was not observed a bias potential of proportion (P>0.05). There was not a systematic inclination of the methods to overestimate or underestimate errors. This methodology can be carried out with roughage and concentrate feedstuffs simultaneously.

Highlights

The concentration of structural carbohydrates has been used to determine the nutritional and digestible quality of food, as well as being a predictor in mathematical models to estimate energy
The difference in Neutral detergent fiber (NDF) and acid detergent fiber (ADF) values was evaluated between the non-pressurized environment and the two temperatures in a pressurized environment (Table 2)
In the NDF analysis, we observed a significant difference between the pressurized at 100 °C and nonpressurized environments (P

Summary

Introduction

The concentration of structural carbohydrates has been used to determine the nutritional and digestible quality of food, as well as being a predictor in mathematical models to estimate energy. The fiber detergent analysis system was initially proposed by Van Soest in 1963 to determine neutral detergent fiber (NDF) and acid detergent fiber (ADF). The proposed w to apply this methodology in forage, it can be extended to analyze concentrated foods. Is well know that starch can contaminate samples, causing an overestimation of fiber values (Van Soest et al, 1991). The method to determine NDF and ADF has been improved to reduce the amounts of starch by using α-amylase, sodium sulfite, or 8M urea solution (Van Soest et al, 1991). Other adaptations have been proposed as an alternative to the original test, including an application of the filter bag procedure and a replacement of the conventional digester such as the use of pressurized equipment, e.g., an autoclave. (Pell and Schofield, 1993; Deschamps, 1999; Ferreira and Mertens, 2007; Senger et al, 2008)

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