Abstract
Pyroptosis, a kind of pro-inflammatory programmed necrosis, can be caused by pathogens. Edwardsiella piscicida is a common pathogenic bacterium, which can infect several teleosts including black rockfish (Sebastes schlegelii), leading to serious disease. However, the interaction mechanism between E. piscicida and immune cells in S. schlegelii is not clear. Herein, we showed that upon the stimulation with E. piscicida, head kidney macrophages (HKMs) exhibited remarkable pyroptosis phenomena, including evident cell swelling, membrane rupture, DNA damage, and cleavage of SscGSDMEb. Furthermore, the transcriptome profiles of HKMs infected with E. piscicida were examined. A total of 198 differentially expressed genes (DEGs) were identified and significantly enriched in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to immunity. The expression of several pyroptosis-associated genes changed significantly after E. piscicida infection. Moreover, we found reactive oxygen species (ROS) was essential in pyroptosis and inflammatory response induced by E. piscicida. Together, we revealed that E. piscicida invasion caused pyroptosis in HKMs of S. schlegelii, key genes and pathways in pyroptosis were obtained. This study could prove novel insights into the immune mechanism underlying the resistance of teleosts to pathogen invasion.
Published Version
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