Abstract
Stress is known to induce enhanced activity of the pituitary-adrenal axis, resulting in increased secretion of corticosteroids from the adrenal cortex. The plasma level and urinary excretion of corticosteroids have been measured for assessment of stress. It has been demonstrated that corticosteroids in plasma are largely bound to proteins, mainly corticosteroid-binding globulin, and that these proteinbound corticosteroids are biologically inactive, whereas the non-bound fraction is active [ 11. According to Beisel et al. [ 21, the level of urinary free corticosteroid reflects that of the non-bound fraction in plasma, because the free fraction in plasma is filtered out in the kidney whereas the protein-bound fraction is not. It is assumed, therefore, that the urinary free corticosteroid level is a useful index for evaluation of stress in various situations. Our preliminary report [ 31 described a high-performance liquid chromatographic (HPLC) method for the determination of cortisol in human plasma or saliva. The aim of the present work was to apply this method for measurement of corticosterone in urine and plasma of rats under both basal states and stress situations. For this purpose, the conditions of HPLC and the post-column reaction were optimized and recovery throughout the procedure was corrected using an internal standard.
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