Analysis of correlation between RASSF2A gene methylation and colorectal cancer

Abstract

Objective To investigate the methylation status of RASSF2A gene promoter in colorectal cancer tissues and its correlation with the occurrence of colorectal. Methods From February 2007 to March 2013, a total of 111 cases of sporadic colorectal cancer patients were selected. The methylation status of RASSF2A gene in tumor tissue and normal mucosa were detected by using combined bisulfite restriction endonuclease method (COBRA). The correlations between the methylation of RASSF2A gene and the clinicopathologic features of colorectal cancer were analyzed. Results Methylation of RASSF2A gene was found in 83.78% (93/111) and 7.21% (8/111) of colorectal cancer tissues and normal tissues, with statistically significant difference (χ2=131.245, P<0.001). The methylation of RASFF2A gene was not correlated with histology type (χ2=3.554, P=0.314), Duke staging (χ2=3.217, P=0.359) and location of tumor (χ2=1.060, P=0.303). Conclusion The presence of RASSF2A hypermethylation in colorectal cancer may be associated with the occurrence of colorectal cancer. Key words: Colorectal neoplasms; DNA methylation; RASSF2A gene