Study on the correlation between Ras related sequence family 2A gene methylation and colorectal cancer in feces

Abstract

Objective To investigate the value of methylation of Ras related sequence family 2A (RASSF2A) gene methylation in the stool of patients with colorectal cancer in the clinical diagnosis of RASSF2A. Methods A modified COBRA method was used to analyze the methylation status of 102 RASSF2A gene promoter regions (region 1 and region 2) in two cases of colorectal cancer. 51 cases of healthy subjects as control group negative colonoscopy. At the same time, the relationship between the methylation of RASSF2A gene and clinical pathological factors. Results In the stools of patients with colorectal cancer extensive methylation, methylation and methyl of were 87% and 63% (64/102) 89/102 and 13% (13/102) and 6% (3/51) and 22% (11/51) and 78% (40/51) in the control group respectively. The detection rate of methylation in stool of colorectal cancer patients was significantly higher than that of healthy persons, and the rate of non methylation was significantly lower than that of the control group, the difference was statistically significant (P=0.000). There was no significant correlation between the methylation of RASSF2 gene and the gender, tumor stage, and the degree of invasion of colorectal cancer patients. Conclusion Methylation of RASSF2A gene is an early event in the progression of colorectal cancer. Methylation of RASSF2 gene in feces is expected to be a new approach to the early diagnosis of colorectal cancer. Key words: Ras related sequence family 2A gene; Methylation; Colorectal cancer