Analysis of composition of the neutral fraction of the oleoresin of Abies sibirica using a scheme of group separation

Abstract

Information has been published on the composition of the acid fraction [I-3], the volatile components [4, 5] and the neutral diterpenoids [i, 6-8] of the oleoresin of the Siberean fir Abies sibirica Ledb., which is produced on the industrial scale. In view of the tendency of one of its components cis-abienol [i, 6] to undergo isomerization [9, I0] and polymerization [I], the facts relating to the neutral diterpenoids require refinement under conditions of analysis excluding the use of high-temperature facuum distillation. We have separated the neutral fraction of the oleoresin collected in the Maslyanino region of Novosibirsk province by the scheme of the group separation of conifer oleoresins [ii]. In this way we obtained fractions of hydrocarbons (40%; here and below, on the neutral part), of carbonyl compounds and oxides (22%), of tertiary nonpolar alcohols (31%), of polar monohydric alcohols (3%), and of polyfunctional compounds (3%). In addition, we isolated a small fraction (1%), eluted before the combined nonpolar alcohols, that consisted of a mixture (i:I) of caryophyllene s-oxide [5] and 6,7-epoxyhumulene [12], which were identified from their PMR spectra after their separation.