Analysis of Biological Particles by Mass Spectrometry

Abstract

Abstract : A new method has been developed for the rapid characterization of aerosol particles of biological nature using the technique of Particle Analysis by Mass Spectrometry (PAMS). PAMS is a combination of particle beam generation with mass spectrometry. Mass spectral measurements were made on individual biological aerosol particles using this method. The generation of mass spectra directly from aerosols on a single particle basis makes PAMS a unique method for the detection and identification of biological particles in air on a instantaneous basis. Aerosols of various organic compounds, and microorganisms were produced by nebulizing their suspensions, and were introduced into the ion source of a quadrupole mass spectrometer in the form of a beam. It is found that the particle beam method provides a well-controlled means for introducing one particle at a time into the ion source. Two methods for the volatilization and ionization of particles were studied: thermal volatilization followed by electron impact ionization, and laser-induced volatilization and ionization. Originator furnished keywords include: Particle analysis by mass spectrometry (PAMS); Individual biological particle; Pyrolysis-mass spectrometry (Py-MS); Particle beam; Aerosols; Bacteria particle mass spectra; Beam generator; Volatilization and ionization; Continuous, real-time detection.