Abstract
Objective: The present work was done to find out various bioactive compounds present in the chloroform extract of Lycoperdon pyriforme using gas chromatography-mass spectrometry (GC-MS).
 Methods: L. pyriforme was collected cleaned to remove any dirt and oven dried at 60°C. The sample was then finely powdered and extracted with chloroform using Soxhlet apparatus. The chemical composition of chloroform extract was then analyzed using QP2010S-Shimadzu GC-MS instrument.
 Results: Chloroform extract resulted in the presence of 33 compounds with 1-Heneicosanol (11.17%) and E-15-Heptadecenal (11.08%) forming major compounds and 1-Tetradecanol (0.16%), Dichloroacetic acid, and decyl ester (0.15%) forming least.
 Conclusion: The results indicated the presence of a variety of compounds thus providing the information about various bioactive compounds present in Lycoperdon sp. and its further application in the field of pharmacology.
Highlights
Fungi besides being pathogens are known to possess medicinal values
gas chromatography-mass spectrometry (GC-MS) analysis of chloroform extract of L. pyriforme showed the presence of 33 different compounds
About 33 compounds have been found in which 1-Heneicosanol (11.17%) and E-15-Heptadecenal (11.08%) are the major compounds and Dichloroacetic acid, decyl ester (0.15%), and 1-Tetradecanol (0.16%) formed minor compounds in the chloroform extract
Summary
Visually distinctive compared with other class of fungi with naked fruiting bodies, have been used in traditional medicines as “the ultimate health food” [1]. Their medicinal characteristics have led them to be used in the field of drug development. Lycoperdon otherwise “puffballs” a saprobe belongs to the family Agaricaceae. They are widely distributed in tropics growing on various habitats, on tree stumps, on decaying logs, and on ground among fallen leaves [6]. The present work has been carried out to understand various bioactive compounds present in Lycoperdon pyriforme In the present study, chloroform extract has been used and various compounds are detected using gas chromatography-mass spectrometry (GC-MS) technique
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