Analysis of association between genetic polymorphism of long non-coding RNA ANRIL and survival of patients with genitourinary cancer

Abstract

Objective – to study the possible association of kidney cancer and bladder cancer onset with rs4977574-polymorphism of long non-coding RNA ANRIL gene depending on patients age.Material and methods. Whole venous blood of 242 persons with genitourinary cancer (101 patients with clear cell renal cell carcinoma and 141 patients with transitional cell carcinoma of urinary bladder) was used for the study. Genotyping of ANRIL gene rs4977574 polymorphism was performed by real-time polymerase chain reaction (Real-time PCR) in the presence of TaqMan assay C_31720978_30. The data were statistically processed using the SPSS software package (version 17.0).Results. It was found that malignant neoplasms of the urogenital tract in G-allele carriers of ANRIL gene rs4977574 polymorphic site occur approximately 5 years earlier, than in AA-homozygotes (log rank P = 0.022; Breslow P = 0.006). The results of Cox regression analysis under dominant model of inheritance showed that G-allele carriers have higher risk of genitourinary cancer (HR = 1,369; P = 0,030) compared to main A-allele homozygotes. The reliability of results was maintained even after adjustment for sex, body mass index, metastases presence, smoking and alcohol consumption (HR = 1,348; P = 0,040).Conclusion. The risk of genitourinary cancer development in G-allele carriers of ANRIL gene rs4977574-polymorphism is higher, compared to AA-homozygotes.