Abstract
Objective: The aim of this study was to analyze the chemical composition of the hydroalcoholic extract of Anacardium occidentale L. (cashew) and the antimicrobial activity of this antibiotic against resistant gram-negative bacteria (GNB). Theoretical Framework: The spread of resistant microorganisms to available chemotherapeutic drugs is a major public health problem and points to the need for research into new antimicrobials. Plants, due to their secondary metabolism, produce compounds of biological importance, being an important source of bioprospection. Method: The collected leaves were dried at 37 °C, ground and soaked in 70% ethanol-water solution at a ratio drug / solvent of 1: 5 for seven days. After this time the mash was filtered and dried at 40 °C in a water bath until completely dry and obtain of the dry extract. The phytochemical analysis was based on the qualitative determination of saponins, alkaloids, flavonoids, steroids / triterpenoids and tannins. The study of the susceptibility of the BGN extract was determined by the technique of REMA (Resazurin Microtiter Assay), using the minimum inhibitory concentration (MIC). The maintenance of the blue or pink color in the wells of the plate was interpreted, respectively, as the presence and absence of microbial growth. Results and Discussion: Phytochemicals in trials observed the presence of saponins, flavonoids and tannins and analysis of antimicrobial activity results demonstrated weak antimicrobial activity against most of the microorganisms evaluated, except for Pseudomonas sp. (7370) that showed MIC of 250µg/mL. Research Implications: Quantitative methods are necessary to better assess and quantify the secondary metabolites of the Anacardium occidentale L. in the region.
Published Version
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