Analysis and significance of K-ras gene mutation in intrahepatic cholangiocarcinoma

Abstract

Objective To detect the K-ras gene mutation in intrahepatic cholangiocarcinoma by direct sequencing, and explore the relationship between K-ras gene mutation and pathogenesis of intrahepatic cholangiocarcinoma. Methods The genomic DNA was extracted from paraffin tissue sections of 32 cases of intrahepatic cholangiocarcinoma and 12 cases of intrahepatic bile duct benign lesions, respectively. K-ras 2 exon fragments were amplificed by PCR and their mutation status was detected by direct sequencing. Results 24 cases were found The K-ras gene mutation was found in 24 out of 32 patients with intrahepatic cholangiocarcinoma, and no K-ras gene mutation was detected in 12 benign lesions with the difference being significant (χ2=14.570, P=0.000). There were 22 cases with codon 12 mutation (91.7%), 1 case with codon 61 mutation, and 1 case with codon 23 mutation. Conclusion The mutation of the K-ras gene may be closely related to the pathogenesis of intrahepatic cholangiocarcinoma. Key words: Intrahepatic cholangiocarcinoma; K-ras; Gene mutation