Abstract
Objective: To evaluate the quality of Pueraria lobata sold in the market and establish a HPLC method for the analysis of Pueraria lobata. Method: Acclaim 120 C18 chromatographic column (5 μm. 4.6 * 250mm), acetonitrile-0.1% formic acid aqueous solution was used as the mobile phase for gradient elution, and the contents of 5 components, 3'-methoxy puerarin, puerarin, daidzin, daidzein, and 3'-hydroxypuerarin, were determined in 11 commercial pueraria lobata from different habitats. Results: The contents of these five components in pueraria lobata from different habitats were different. Anhui No. 003 and No. 006 have higher chemical constituents. Conclusion: The method established in this experiment is accurate, with good separation effect, and can be used to detect the content of five different substances in Pueraria lobata. The determination results can provide valuable reference information for the purchase of traditional Chinese medicine stores and provide effective scientific basis for the development of Pueraria lobata resources.
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