Analysis and Construction of a Competitive Endogenous RNA Regulatory Network of Baicalin-Induced Apoptosis in Human Osteosarcoma Cells.

Haifeng Lan,Mi Gao,Dongyang Qian,Guan Luo,Qinghua Wu,Chunxiao Liang,Xiaoxiao Xiong,Wei Hong,Xing Chen,Jiahuan Zhang,Biting Lin,Erkang Yi,Haiyan Wang,Ruikun Chen,Yang Deng

doi:10.1155/2021/9984112

Abstract

Background Baicalin is an extract from the traditional Chinese herb Scutellaria baicalensis and has the potential to treat osteosarcoma (OS). However, the transcriptome-level mechanism of baicalin-mediated antitumor effects in OS has not yet been investigated. The aim of this study was to analyze the competitive endogenous RNA (ceRNA) regulatory network involved in baicalin-induced apoptosis of OS cells. Methods In this study, CCK-8 and flow cytometry assays were used to detect the antitumor effects of baicalin on human OS MG63 cells. Furthermore, transcriptome sequencing was employed to establish the long noncoding RNA (lncRNA), microRNA (miRNA), and mRNA profiles. Results Baicalin inhibited MG63 cell proliferation and induced apoptosis. Totals of 58 lncRNAs, 31 miRNAs, and 2136 mRNAs in the baicalin-treated MG63 cells were identified as differentially expressed RNAs compared to those in control cells. Of these, 2 lncRNAs, 3 miRNAs, and 18 mRNAs were included in the ceRNA regulatory network. The differentially expressed RNAs were confirmed by quantitative real-time PCR (qRT-PCR). Conclusions By identifying the ceRNA network, our results provide new information about the possible molecular basis of baicalin, which has potential applications in OS treatment.

Highlights

Osteosarcoma (OS) is the most common primary bone tumor with high mortality and poor prognosis [1, 2]
The human OS cell line MG63 was purchased from the American Type Culture Collection (ATCC, VA, USA; CRL-1427TM) and cultured in the recommended medium supplemented with 10% fetal bovine serum (FBS; Gibco, USA) at 37°C in a humidified atmosphere with 5% CO2
Our results showed that baicalin significantly inhibits the proliferation of human OS cells

Summary

Introduction

Osteosarcoma (OS) is the most common primary bone tumor with high mortality and poor prognosis [1, 2]. Baicalin is an extract from the traditional Chinese herb Scutellaria baicalensis and has the potential to treat osteosarcoma (OS). The aim of this study was to analyze the competitive endogenous RNA (ceRNA) regulatory network involved in baicalin-induced apoptosis of OS cells. Transcriptome sequencing was employed to establish the long noncoding RNA (lncRNA), microRNA (miRNA), and mRNA profiles. Totals of 58 lncRNAs, 31 miRNAs, and 2136 mRNAs in the baicalin-treated MG63 cells were identified as differentially expressed RNAs compared to those in control cells. 2 lncRNAs, 3 miRNAs, and 18 mRNAs were included in the ceRNA regulatory network. By identifying the ceRNA network, our results provide new information about the possible molecular basis of baicalin, which has potential applications in OS treatment

Objectives

Methods

Discussion

Conclusion