Analisis Sekuen Gen Tubulin-β Isotipe 1 Cacing Haemonchus contortus Isolat Resisten terhadap Benzimidazole pada Domba di Indonesia

Abstract

<p>Benzimidazole (BZ)<br />resistance to gastrointestinal nematodes in small ruminants<br />(sheep and goat) has become a significant problem worldwide.<br />Evidences of anthelmintic resistance to albendazole in<br />Indonesia has been reported from some government owned<br />farms in West Java, Central Java, and Yogyakarta. Previous<br />study on the sheep parasite H. contortus had shown that the<br />BZ resistance was related to selection for individuals in a<br />population possesing a spesific β-tubulin isotype 1 gene. The<br />study is aimed to determine mutation on coding region of<br />central part of β-tubulin isotype 1 gene of H. contortus resistant<br />strain from Indonesia. Seven H. contortus worms were<br />isolated from four BZ resistant sheep from two government<br />farms (SPTD Trijaya, Kuningan, West Java, and UPTD<br />Pelayanan Kesehatan Hewan, Bantul, Yogyakarta), and from<br />a BZ susceptible sheep from Cicurug, Sukabumi, West Java.<br />DNA was extracted individually from female H. contortus<br />worms. A fragment of 520 bp β-tubulin isotype 1 gene exon<br />3, 4, 5 was amplified using the PCR technique and then<br />sequenced. The results showed that a single mutation<br />occurred in codon 200 (from phenilalanine to tyrosine) had<br />caused benzimidazole resistance in H. contortus from SPTD<br />Trijaya, Kuningan, West Java. Mutation in β-tubulin isotype 1<br />gene of H. contortus from UPTD Pelayanan Kesehatan<br />Hewan, Yogyakarta, occurred in codon 198 (from glutamate<br />to glycine), codon 201 (from cystein to stop codon), and<br />codon 202 (from isoleucyne to stop codon).</p>