Abstract
Background: Bacterial contamination in thrombocyte concentrate (TC) blood product becomes a serious problem since it can cause clinical risks from mild symptom to death. TC storage condition at 22-25oC with agitation is good condition for bacterial growth. Source of bacterial contamination can come from donor bacteremia, not aseptically from taking and processing blood product. In a previous study, showed there were positive gram bacteria as bacterial contamination in TC.
 Objective: This objective aims to determine bacterial contaminants in TC blood products. 
 Methods: Total of 3 TC bag samples were collected from UDD PMI Kabupaten Sleman. Each sample was inoculated to nutrient agar media and it incubated for 48 jam at 37oC. Observation of bacterial colony growth is carried out after incubation time is over.
 Results: There was no bacterial growth in the media so that there was no bacterial contamination in TC. It showed that the procedures, processing, and storage of TC products in PMI Kabupaten Sleman were aseptic. Besides that, TC donors also did not suffer from bacteremia so there was bacterial contamination in TC.
 Conclusion: In this study, bacterial contamination in blood product TC was not found since there was no bacterial growth in media. 
 Keywords: Bacterial contamination, blood products, blood bank, thrombocyte concentrate.
Highlights
Bacterial contamination in thrombocyte concentrate (TC) blood product becomes a serious problem since it can cause clinical risks from mild symptom to death
Source of bacterial contamination can come from donor bacteremia
Total of 3 TC bag samples were collected from unit donor darah (UDD) PMI Kabupaten Sleman
Summary
Transfusi Darah Palang Merah Indonesia Kabupaten Sleman dan Laboratorium Mikrobiologi Pusat Studi Pangan dan Gizi Universitas Gadjah Mada Yogyakarta pada bulan Juli sampai September 2018. Produk darah TC yang dipilih untuk digunakan sebagai sampel telah disimpan selama 3-5 hari pada suhu ruang (22-25oC) supaya apabila terdapat kontaminasi bakteri maka bakteri yang tumbuh akan semakin banyak(3). Apabila produk darah TC mengandung bakteri kontaminan maka akan berisiko terhadap pasien atau resipien karena terdapat potensi risiko transfusi yaitu bacterial sepsis yang memiliki gejala seperti muntah, syok septik, tachycardia, hipotensi, rigor, dan gejala potensial lain.[9] Berdasarkan penelitian yang dilakukan, tiga kantong sampel produk darah TC diperoleh dari UDD PMI Kabupaten Sleman telah disimpan sesuai dengan standar penyimpanan TC yaitu pada suhu 22-25oC di atas agitator dengan masa simpan sampai dengan 5 hari pada kantong. Berdasarkan hasil penelitian yang dilakukan diperoleh bahwa setelah dilakukan inokulasi dan inkubasi pada kultur sampel produk darah TC pada media NA tidak menunjukkan adanya pertumbuhan bakteri dengan tidak adanya koloni yang tumbuh di atas media NA (Gambar 2). Selain itu juga menunjukkan bahwa proses pemisahan TC yang dilakukan pada UDD PMI Kabupaten Sleman dilaksanakan secara aseptis dan peralatan yang digunakan dipastikan steril
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