Abstract

Analyze of acidity and amount of lactic acid bacteria of yogurt made from different milk types and lactobacillus casei percentageABSTRACT. The objective of this experiment is to determine the effect of milk types and percentages of Lactobacillus casei as a starter to the amount of Lactic Acid Bacteria in yogurt and its pH value and Lactic Acid value. Factorial Completely Randomized Design with two factors and three replications was applied. The A factor was the milk types that were a1 = Powder Milk, a2 = Fresh Milk and a3 = UHT milk. The B factor was the percentages of Lactobacillus casei (b1 = 5% and b2 = 10%). Parameters observed were the level of lactic acid, the pH value of yogurt and the amount of lactic acid bacteria. The result showed significantly difference (P0.01) from the use of milk types and Lactobacillus casei percentages on the lactic acid level, and there are no interaction between factors. There was also significant different (P0.01) showed from pH value and an interaction between both factors was presented. In addition there was significant difference (P0.05) showed by the amount of lactic acid bacteria, and an interaction between both factors was also existed. The research concluded that the higher the percentage of starter, the higher the level of lactic acid and the amount of lactic acid bacteria of yogurt that made from all milk types. On the contrary, the higher the percentage of starter, the lower the pH value of yogurt.

Highlights

  • Susu merupakan bahan makanan yang istimewa karena kelezatannya dan komposisinya yang seimbang

  • The objective of this experiment is to determine the effect of milk types and percentages of Lactobacillus casei as a starter

  • The B factor was the percentages of Lactobacillus casei

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Summary

MATERI DAN METODE PENELITIAN

Penelitian ini dilaksanakan di Laboratorium Pengolahan Susu Jurusan Peternakan Fakultas Pertanian Universitas Syiah Kuala. Materi yang digunakan adalah Susu Segar (Kadar lemak 3,9%, Protein 3,4% dan Laktosa 4,8%), Susu UHT (Kadar lemak 3%, Protein 3% dan Laktosa 5%), Susu Bubuk Full cream instan untuk dewasa Kadar Lemak 3,24%, Protein 3,24% dan Laktosa 5,09%), dan bakteri Lactobacillus casei sebagai Starter. Susu bubuk dilarutkan dengan air yang telah disterilkan, sedangkan susu UHT langsung digunakan untuk pembuatan yogurt tanpa dilakukan perlakuan apapun. Kandungan nutrisi masing-masing susu sebagi berikut Parameter yang diamati adalah Total Bakteri Asam Laktat dengan menggunakan metode plate Count dan memakai media M.R.S (de man, Rogosa, Sharpe) Agar. Data yang diperoleh diolah dengan menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan dua faktor yaitu : faktor A jenis susu yaitu a1 = susu segar, a2 = susu pasturisasi, a3 = susu bubuk dan faktor B adalah persentase starter Lactobacillus casei yaitu, b1 = 5% , b2 = 10%. Maka dilakukan pengujian dengan menggunakan Uji Jarak Berganda Duncan (Steel dan Torrie, 1993)

HASIL DAN PEMBAHASAN
Persentase Starter
Semakin tinggi penambahan starter semakin tinggi kadar asam laktat dan jumlah
Findings
DAFTAR PUSTAKA
Full Text
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