Analgesic, Anti-Inflammatory, and Chondroprotective Activities ofCryptolepis buchananiExtract:In VitroandIn VivoStudies

Nutthiya Hanprasertpong,Narong Nathasaen,Chaichan Sangdee,Ampai Panthong,Siriwan Ongchai,Samreang Bunteang,Vichai Reutrakul,Puongtip Kunanusorn,Supanimit Teekachunhatean,Rujirek Chaiwongsa

doi:10.1155/2014/978582

Nutthiya Hanprasertpong, Narong Nathasaen + Show 8 more

Open Access

https://doi.org/10.1155/2014/978582

Copy DOI

Abstract

Cryptolepis buchanani Roem. & Schult. is widely used in folk medicine in Southeast Asia for treating muscle tension and arthritis. This study aimed to investigate an analgesic activity of the methanol extract of C. buchanani (CBE) in acetic acid-induced writhing response in mice, and to examine its anti-inflammatory activity in ethyl phenylpropiolate- (EPP-) induced ear edema and carrageenan-induced paw edema in rats. Its effects on cartilage degradation induced by interleukin-1β (IL-1β) in porcine cartilage explant culture were also determined. This study demonstrated that CBE significantly reduced acetic acid-induced writhing response. It also inhibited edema formation in both EPP-induced ear edema and carrageenan-induced paw edema models. In cartilage explant culture, CBE significantly reduced the sulfated glycosaminoglycan and hyaluronan released into culture media while it reserved the uronic acid and collagen within the cartilage tissues. It also suppressed the matrix metalloproteinase-2 activity with no effect on cell viability. In conclusion, CBE shows analgesic, anti-inflammatory, and chondroprotective effects in this preliminary study. Therefore, CBE may be useful as an alternative treatment for osteoarthritis.

Highlights

Osteoarthritis (OA) is a chronic degenerative disorder characterized by destruction of articular cartilage and periarticular bone remodeling [1]
Indomethacin, chondroitin sulfate C, D-glucuronic acid lactone, hyaluronan, matrix metalloproteinase-2 (MMP-2), and recombinanthuman interleukin-1β were obtained from Sigma
C. buchanani methanolic extract (CBE) significantly reduced the sulfated glycosaminoglycan (s-GAG) and HA released from cartilage explants into the culture media while reserved the cartilage matrix molecules such as uronic acid and collagen

Summary

Introduction

Osteoarthritis (OA) is a chronic degenerative disorder characterized by destruction of articular cartilage and periarticular bone remodeling [1]. In OA, degradation of extracellular matrix component and consequent gradual articular cartilage destruction is caused by an increase in cartilage catabolism and a decrease in cartilage anabolism [4]. This cartilage pathology is often accompanied by pain and joint dysfunction. In Thailand, C. buchanani known as “Thao En On” has been used for treating inflammatory conditions such as muscle and joint pain [7,8,9,10]. The objectives of this study were to evaluate the analgesic and anti-inflammatory activities of C. buchanani methanolic extract (CBE) in animal models and its chondroprotective effect in cartilage explants

Materials and Methods

Results

Discussion

Conclusion