Abstract
Objectives To study the quantification of the components in rat plasma after oral administration of Shenyanyihao oral solution. Methods Shenyanyihao oral solution has been traditionally used for the treatments of chronic nephritis in clinics. Stachydrine, Danshensu, chlorogenic acid, protocatechuic acid, plantamajoside, aesculetin, isoquercitrin, ferulic acid, baicalin, and baicalein are regarded as the main compounds in Shenyanyihao oral solution. A sensitive, efficient, and precise UPLC-MS/MS method was established and validated for the quantification of the components in rat plasma after oral administration of Shenyanyihao oral solution. Results The main pharmacokinetic parameters of the components were acquired based on the analysis of the plasma sample by a noncompartmental method using the WinNonlin7.0 pharmacokinetic program. Danshensu, protocatechuic acid, isoquercitrin, and ferulic acid from Shenyanyihao oral solution were quickly absorbed, and their peak concentration occurred at less than 0.5 h. The pharmacokinetic parameter of the average t1/2 from Danshensu was 3.91 h in rats, and it was the most rapid distribution and elimination among the components. In addition, the Cmax of stachydrine and baicalin were revealed as the higher plasma concentrations in rats. Conclusions This pharmacokinetic study seems to be useful for a further clinical study of Shenyanyihao oral solution in the treatments of chronic nephritis.
Highlights
Shenyanyihao oral solution, a famous traditional Chinese preparation, was created by experts in traditional Chinese medicine from the school of Wumen based on the pathological characteristics of chronic nephritis, and it is the internal preparation of the Traditional Chinese Medicine Hospital of Suzhou province, China
Pure acetonitrile was utilized as the precipitant for biosamples
Different mobile phases were evaluated to improve LC separation and enhance mass sensitivity of analytes. Modifiers such as formic acid and ammonium acetate were added with different concentrations
Summary
Shenyanyihao oral solution, a famous traditional Chinese preparation, was created by experts in traditional Chinese medicine from the school of Wumen based on the pathological characteristics of chronic nephritis, and it is the internal preparation of the Traditional Chinese Medicine Hospital of Suzhou province, China. It has been used clinically for nearly two decades and is demonstrated to be efficient in improving the clinical symptoms and reducing proteinuria in patients with chronic nephritis [1]. Previous research demonstrated that Rhizoma Atractylodis Macrocephalae and its compounds could exert immunoregulation, BioMed Research International
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