Abstract
BackgroundCyclocarya paliurus (Batal.) Ijinskaja (CP) is a monotypic genus plant, also called sweet tea tree that belongs to the Juglandaceae family, which is mainly distributed in the subtropical highlands in China. Our previous work has verified that CP leaves exhibit a potent hyperglycemic effect by inhibiting pancreatic β cell apoptosis through the regulation of MPAK and Akt signaling pathways. However, the components that contribute to this potential health benefit remain undiscovered.MethodA sensitive, reliable, and validated ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UPLC–TQ-MS/MS) method was developed to simultaneously determine the presence of six active components (neochlorogenic acid, chlorogenic acid, quercetin-3-O-glucuronide, kaempferol-3-O-rhamnoside, quercetin, and kaempferol) in rat plasma after a single oral administration (in a dosage of 10.5 g/kg) of an extract of CP leaves to rats. The separation was performed on a Waters ACQUITY BEH C18 column (50 mm × 2.1 mm, 1.7 μm). The detection was conducted by multiple reaction monitoring (MRM) in negative ionization mode. The two highest abundant MRM transitions without interference were optimized for each analyte. Acetonitrile and formic acid aqueous solution (0.1%) was used as the mobile phase at a flow rate of 0.3 ml/min.ResultThe precision, accuracy, and recovery all satisfied the criteria of international guidance (Bioanalytical Method Validation Guidance for Industry, Food and Drug Administration), and the analytes were stable in plasma for all tested conditions. The main pharmacokinetic parameters were calculated by plasma concentration versus time profiles using the pharmacokinetics program.ConclusionThe pharmacokinetic parameters of each compound can facilitate future clinical studies.
Highlights
Cyclocarya paliurus (Batal.) Ijinskaja (CP) is a monotypic genus plant that belongs to the Juglandaceae family, which is mainly distributed in the subtropical highlands in China [1]
It has been reported that quercetin-3-O-glucuronide is as effective as quercetin in ameliorating insulin resistance by regulating the IRS-1 function of endothelium [14]; kaempferol-3-O-rhamnoside can significantly stimulate GLUT-4 translocation and synthesis in adipocytes [15]; and kaempferol-3-O-glucopyranoside possesses potent α-glucosidase inhibitory activity [16, 17]
Quinic acid, gallic acid, kaempferol-3-O-glucopyranoside, asiatic acid, and loganin-7-O-pentoside were found in rather low concentrations in the CP extract; in contrast, neochlorogenic acid, chlorogenic acid, quercetin-3-O-glucuronide, kaempferol-3-O-rhamnoside, quercetin, and kaempferol are the main compounds with high concentration in the CP extract [4]
Summary
Cyclocarya paliurus (Batal.) Ijinskaja (CP) is a monotypic genus plant, called sweet tea tree that belongs to the Juglandaceae family, which is mainly distributed in the subtropical highlands in China. We analyzed the absorbed components of the CP extract in rat plasma by using UPLC-Q-TOF/MS; 13 absorbed components were identified—quinic acid, gallic acid, neochlorogenic acid, chlorogenic acid, p-hydroxybenzoid acid, quercetin-3-O-glucuronide, kaempferol-3-O-glucopyranoside, kaempferol-3-O-rhamnoside, quercetin, kaempferol, quadranoside IV, asiatic acid-, and loganin-7-O-pentoside [1] These compounds can be classified into three groups—organic acids, flavonoids, and triterpenes; some of these compounds have been reported to have potent beneficial effects in the treatment of diabetes. No reports are available on the simultaneous determination and pharmacokinetic studies of the abovementioned main active components in the CP extract Among these compounds, quinic acid, gallic acid, kaempferol-3-O-glucopyranoside, asiatic acid, and loganin-7-O-pentoside were found in rather low concentrations in the CP extract; in contrast, neochlorogenic acid, chlorogenic acid, quercetin-3-O-glucuronide, kaempferol-3-O-rhamnoside, quercetin, and kaempferol are the main compounds with high concentration in the CP extract [4]. The results of this study would be helpful for improving clinical therapeutic efficacy and for further pharmacological studies on CP leaves
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
