An undergrad experiment for the at-home study of fluorescence: Extraction of quinine and chlorophyll from Cinchona tree bark

Abstract

Herein we describe a method for the effective extraction and qualitative detection of quinine and chlorophyll. Both compounds can be successfully extracted from Cinchona tree bark (Cinchona pubescens Vahl) using nothing but household materials, usually cheap and easily acquired items available in any hardware store. During solid-liquid extraction with reflux, using an organic solvent is used to retain both compounds. Afterward, the compounds are partitioned between water and paint solvent. The organic phase containing the chlorophyll is saved, and the aqueous layer is acidified. Finally, an ultraviolet (UV) light is used to provoke fluorescence in both compounds.