Abstract
Using phage display technology, a novel mouse scFv phage clone D1 was generated from mice immunized with single-chain HLA-A2, the human MHC class I molecules. D1 recognized a determinant in β2-microglobulin. It reacted with β2-microglobulin associated with the heavy chain of HLA-A2, recombinant β2-microglobulin made in Escherichia coli or β2-microglobulin prepared from human urine. The heavy chain variable region of D1 belongs to gene family VII and the light chain variable region belongs to the kappa chain gene family XI according to the Kabat database.
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