Abstract
Micro- and nanomechanical analytical devices for diagnostics require small sample volumes, and stable and efficient clamping of the sensors for optimized bioassays. A fully automated device for the readout of the dynamic and static response of cantilevers in a physiological liquid environment including a sample handling system is presented. The device provides sequential readout of the static and dynamic mode providing the best signal to noise ratio for each individual cantilever. In the dynamic mode, it is possible to measure up to the 16th flexural resonance mode of vibration of a 500 μm long and 1 μm thick cantilever. The automated sample handling system enables the local injection of sub microliter volumes of sample with excellent reproducibility. Data demonstrating the response of the cantilevers to external stimuli highlight the sensitivity of the device and the importance of the use of reference cantilevers to decouple biologically relevant metrics from environmental effects. A specific biomolecular interaction of sensitised nanospheres on cantilever sensors with a mass resolution of ~50 picogram in liquid is shown. PACS numbers: 07.10 Mechanical instruments, 87.85 Biosensors, 47.85. Fluidic
Highlights
Cantilever technology has been used for a wide variety of applications including mass, force and biological sensing
The obtained laser beam radius was 7.177 μm. This quick method of the laser focusing gave an accurate determination of the laser spot radius, an optimally focused laser on the cantilever for the static and dynamic mode measurements
We investigated the effect of signal amplification and active actuation of the cantilevers with the integrated piezo-electric stack
Summary
Cantilever technology has been used for a wide variety of applications including mass, force and biological sensing. Thermal noise versus active dynamic actuation with the integrated piezo A cantilever array was loaded into the fluid chamber and allowed to equilibrate in deionized water. Flow rate effects A cantilever array was loaded into the fluid chamber and allowed to equilibrate in nanopure water.
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