Abstract
Using previously reported protocols, electroporation of 21 very sensitive human cell lines showed poor results with high mortality and low transfection efficiency. Therefore, the influence of several electroporation parameters on transfection success was analyzed. The adjustment of the time constant proved to be most important for optimization of transfection results. Time constant was modulated by changing medium resistance via volume or ionic strength, yielding an average transfection efficiency of 25% and mortality rates below 60%.
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