Abstract
An open-flow cryostat using helium gas has been developed for cryogenic x-ray crystallography. The device is composed of a cryostat and a heat-exchange apparatus, and dry nitrogen gas is supplied for shielding the cooled helium gas from atmosphere. The lowest temperature reached by the device is 35 K within a fluctuation of ±0.5 K at a flow rate of 6 l/min. This device is easy to operate and enables to study crystal structures and the temperature-dependent phenomena occurring in biological macromolecules and materials above 35 K.
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