An NMR-Based Metabolomic Approach to Unravel the Preventive Effect of Water-Soluble Extract from Dendrobium officinale Kimura & Migo on Streptozotocin-Induced Diabetes in Mice.

Hong Zheng,Linlin Pan,Yongsheng Hu,Pengtao Xu,Jianjun Zhu,Ruohan Wang,Wenzong Zhu,Hongchang Gao

doi:10.3390/molecules22091543

Abstract

Dendrobium officinale Kimura & Migo (D. officinale) is a precious herbal medicine. In this study, we investigated metabolic mechanism underlying the effect of D. officinale water extract (DOWE) on diabetes prevention in mice after streptozotocin (STZ) exposure using NMR-based metabolomics. Interestingly, we found a decrease in blood glucose and an increase in liver glycogen in mice pretreated with DOWE after STZ exposure. The DOWE pretreatment significantly increased citrate and glutamine in the serum as well as creatine, alanine, leucine, isoleucine, valine, glutamine, glutathione and taurine in the liver of STZ-treated mice. Furthermore, serum glucose was significantly negatively correlated with citrate, pyruvate, alanine, isoleucine, histidine and glutamine in the serum as well as alanine and taurine in the liver. These findings suggest that the effect of DOWE on diabetes prevention may be linked to increases in liver glycogen and taurine as well as the up-regulation of energy and amino acid metabolism.

Highlights

Diabetes mellitus (DM), characterized by hyperglycemia due to impaired β-cell function or insulin resistance, is one of the most prevalent chronic metabolic diseases
We suggest that the increase of energy metabolism may be contributed to reduced blood glucose level and diabetes prevention
We found that blood glucose level can be reduced in mice pretreated with D. officinale water extract (DOWE) after STZ exposure, indicating its effect on diabetes prevention

Summary

Introduction

Diabetes mellitus (DM), characterized by hyperglycemia due to impaired β-cell function or insulin resistance, is one of the most prevalent chronic metabolic diseases. A series of complications involving multiple organs can be caused by DM [1], affecting a growing number of people’s health around the world. This number will increase to 642 million in 2040 [2]. It is of great interest and importance to develop a promising strategy for prevention and treatment of DM. Treatment of diabetes using plant extracts has a long history and shows a promising future [3]

Methods

Results

Discussion

Conclusion