Abstract
Three pre-column derivatization reagents, namely o-phthalaldehyde, 4-chloro-7-nitrobenz-2,1,3-oxadiazole, sodium naphthaquinone-4-sulphonate and two ion-pair reagents, namely, naphthalene-2-sulphonate and sodium dodecylsulphate have been investigated for their suitability for the qualitative and quantitative analysis of urine and plasma samples containing amphetamines. The derivatization method employing sodium naphthaquinone-4-sulphonate was found to be selective and sufficiently sensitive for the routine determination of amphetamine and methylamphetamine in urine and plasma samples at the ng/ml level.
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