Abstract
Recent research has focused on ion-permeable channels in cancer, aiming to reduce proliferation and induce apoptosis in cells. Voltage-gated calcium channel blockers [1] and ligand-gated ion/calcium channels [2,3] have been explored. By maintaining low cytoplasmic calcium concentrations at rest and mobilizing calcium in response to stimuli, calcium ions serve as second messengers, regulating diverse cellular stimulus-response reactions [4]. Disruptions in intracellular calcium homeostasis can impact cell proliferation and trigger programmed cell death.In our study, we cultured ovarian cancer (ES-2 cells) on indium tin oxide (ITO)-coated glass as a working electrode. We optimized parameters, including membrane potential amplitude (±60 mV), pulse frequency (1 to 36 times), and duration (2 to 60 seconds). Fig. 1a shows that a single depolarization pulse increases the highest intracellular calcium levels in ES-2 cells. After 24 hours of depolarizing stimulation, the survival rate of ES-2 cells is 66.0±6.2% for single pulses and 74.7±3.7% for multiple pulses. In contrast, the survival rate following hyperpolarization electrical stimulation is 86.3±5.6% for single pulses and 76.5±2.0% for multiple pulses. Fig. 1b indicates that sequential alternating depolarizing and hyperpolarizing stimulation can further induce cell death. Within a possible consideration, the activation of voltage-dependent calcium channels during depolarization stimulation following a stimulation causes calcium influx, and each hyperpolarization stimulation increases permeability of voltage-dependent anion channels to calcium ions between cytosol and mitochondria. This reaction ultimately affects mitochondrial calcium homeostasis and causes cell death. This study of electrical stimulation may have highly possibility for clinical value on therapy method. Reference [1] Int J Mol Sci. 2020 Apr; 21(7): 2327.[2] Front Physiol. 2022 Mar 8;13: 839437.[3] Cancers (Basel). 2020 Aug; 12(8): 2232.[4] J Pharmacol Sci. 2006;100(5):519-24. Figure 1
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call