Abstract
AN INVESTIGATION OF A METHOD FOR IRON DETERMINATION IN BLOOD
Highlights
At present there is no ent,irely satisfactory quantitative method for the determination of iron in whole blood in spite of the fact that numerous methods have been developed
Perhaps the two most widely used methods are the calorimetric thiocyanate and the volumetric tit,anous salt method. There is another successful method for iron determination which involves the use of thioglycolic acid
Hedenstrom and Kunau (1932) and others have shown that there are a large number of substances, many of them found in blood, which produce disturbing effects in the ferric thiocyanate color
Summary
The degree to which the ferric thiocyanate color was decreased by the addition of various substances was measured by Smythe and Schmidt (1930) They found that did inorganic acids such as phosphoric acid and arsenic acid decrease the color produced by a certain amount of iron, and many organic compounds containing a particular grouping which is found in the hydroxymonocarboxylic acids, dicarboxylic acids, and amino acids of the above type. The volumetric titanous salt method involves the quantitative reduction of the ferric ion to ferrous ion When this method is used on small quantities of blood, the end-point of the titration is difficult to ascert,ain if a color indicator is used, and the electrometric method of measuring the end-point, as developed. The purpose of these experiments was to ascertain whether the thioglycolic method when applied to blood gives accurate results
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