Abstract
Bio-plastic synthesis from renewable and cheap agro-based materials is a sustainable solution for replacing conventionally produced plastic with environmental contamination. The current study was aimed at screening and characterization of Polyhydroxyalkanoates (PHA) producing bacterial isolates, evaluation of their potential and recovery of PHA using the isolates. The PHA compounds were characterized using FT-IR. Based on 16SrRNA sequence analyses the isolates were designated as Bacillus sp. BPPI-14 and Bacillus sp. BPPI-19. The isolates were gram-positive, rod-shaped, endospore former, and citrate test positive. Intracellular PHA granules were observed when these isolates were stained with Sudan black B (SBB) and Nile blue A (NBA) preliminary and specific staining dyes, respectively. Effect of pH, temperature and carbon sources on the PHA production by the isolates BPPI-14 and BPPI-19 was studied. Maximum PHA production was recorded for Glucose (49.46±2.79%) by Bacillus sp. BPPI-14 and followed by molasses (45.86±2.17%) by Bacillus sp. BPPI-19, respectively at 37°C and pH7. The obtained PHA polymers were confirmed by preparation of plastic films for both the isolates. Fourier transform infrared spectrum for BPPI-14 and BPPI-19 showed the peak (carboxylic acid group) at 1706–1719.39cm−1 was a characteristic feature of PHA and corresponds functional group (C=O).
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: International Journal of Biological Macromolecules
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.