An Integrated Surfactant Solubilization and PCB Bioremediation Process for Soils

Abstract

Two decades after the manufacture and use of polychlorinated biphenyls (PCBs) were banned, PCB contamination remains widespread in the environment. Technologies available for PCB remediation are limited and often impractical for soils with dispersed PCB contamination. In this study, two remediation processes have been integrated for use on PCB-contaminated soils. This remediation strategy links in situ surfactant washing of PCBs from soil with aerobic biodegradation of the resulting surfactant-PCB solution by two field application vectors (F A Vs), Pseudomonas putida IFL5::TnPCB and Ralstonia eutropha B30F4::TnPCB, which utilize surfac-tants as growth substrates and cometabolize PCBs. A bench-scale demonstration of this process was performed using PCB-contaminated soils from an electric power substation site. In a 2-day recycling wash using a 1% (wt/vol) surfactant solution, greater than 70% of the PCBs were removed from the soil. In the biodegradation phase, greater than 90% of the surfactant and 35% of the PCBs were biodegraded in 12 days. The residual PCBs were partitioned onto a solid carrier resulting in greater than 90% removal of PCBs from the bioreactor effluent and a 50-fold reduction in the amount of PCB-contaminated material.