Abstract
Energy-filtered transmission electron microscopy (EFTEM) has many uses in life sciences1. These include improved contrast for imaging unstained, cryo or thick samples; improved diffraction for electron crystallography, and elemental mapping and analysis. We have developed a new system for biological EFTEM that combines advanced electron-optical performance with a high degree of automation. The system is based on the Philips CM series of microscopes and the Gatan post-column imaging filter (GIF). One combination particulary suited for the life sciences is that of the CM 120-BioTWIN and the GIF100: the CM120-BioFilter. The CM 120-BioTWIN is equipped with a high-contrast objective lens for biological samples. Its specially designed cold-trap, together with low-dose software, supports full cryo-microscopy. The GIF 100 is corrected for second-order aberrations in both images and spectra. It produces images that are isochromatic to within 1.5 eV at 120 keV and distorted by less than 2% over lk x lk pixels. All the elements of the filter are computer controlled. Images and spectra are detected by a TV camera or a multi-scan CCD camera, both of which are incorporated in the filter. All filter and camera functions are controlled from Digital Micrograph running on an Apple Power Macintosh.
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