Abstract
An inexpensive method is presented for preparing thin-section autoradiograms of diffusible substances by freeze-drying and osmium tetroxide vapor fixation of tissue. The unit can be easily constructed, occupies a minimum of space, and should be within the budget of most research laboratories. Both thin sections for light microscopy and ultrathin sections for electron microscopy can be prepared with the apparatus. Due to thinness of sections, the apparatus is best utilized for autoradiographic studies of tissue where there is a significant concentration of the isotope being studied.
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