Abstract
Abstract A high-affinity polyclonal antibody was prepared by immunizing animals with haptens FFD and FFM. Under the optimal combination of coating antigen and antibody, an indirect competitive enzyme-linked immunosorbent assay (icELISA) for simultaneous detection of florfenicol and thiamphenicol residues in animal meat and urine samples was developed. The icELISA showed an IC50 value of 1.32 ng mL−1 for florfenicol and 2.13 ng mL−1 for thiamphenicol, respectively. The linear ranges were from 0.31 to 5.61 ng mL−1 with a limit of detection of 0.12 ng mL−1 for florfenicol, and 0.41 to 11.2 ng mL−1 with a limit of detection of 0.15 ng mL−1 for thiamphenicol, respectively. The average recoveries of florfenicol and thiamphenicol in spiked samples ranged from 77.2% to 116.0% with a relative standard deviation of less than 15%. Therefore, this proposed icELISA provided a valid detection method for florfenicol and thiamphenicol residues in animal tissue and urine samples.
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