Development and validation of an indirect competitive enzyme-linked immunosorbent assay for the detection of albendazole 2-aminosulfone residues in animal tissues

Abstract

ABSTRACTTo monitor the residues of albendazole (ABZ) in edible animal tissues, a monoclonal-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed. The hapten albendazole 2-aminosulfone (ABZSO2NH2) was conjugated to a carrier protein as an immunogen. The spleen cells of the inoculated mice expressing specificity to ABZSO2NH2 were fused. The obtained monoclonal antibody 2A11 was selected for detailed study. The standard curves based on the ABZSO2NH2 matrix calibration ranged from 20.0 to 320.0 µg L−1, with an IC50 value of 85.2 ± 6.3 µg L−1 (n = 5). The limits of detection for ABZSO2NH2 ranged from 11.0 to 20.8 µg kg−1 in edible animal tissues. The recoveries ranged from 73.6% to 99.8% with a coefficient of variation of less than 20%. The developed ic-ELISA exhibited good correlation (r = 0.999) with high-performance liquid chromatography. Thus, it would be a very attractive alternative for the screening of ABZSO2NH2 residues in edible animal tissues.