An Indirect Atomic Absorption Spectrometric Determination of Trifluoperazine Hydrochloride in Pharmaceutical Formulations Based on Chelate Formation with Palladium(II)

Ameen W Qasim,Zuhair A A Khammas

doi:10.1155/2010/153861

Abstract

A new application of an indirect atomic absorption spectrometric (AAS) method was offered for the assay of low concentration of trifluoperazine hydrochloride (TFPH) in pure and pharmaceutical dosage form with good accuracy and precision. The method is depended on the formation of metal complex between the drug (TFPH) and palladium(II) to form orange-yellowish product extractable in organic solvent prior to its aspiration into an air-acetylene flame and indirectly determined by AAS. Using AA responses, all experimental parameters such as, pH, concentration of palladium, reaction time, extraction time and phase ratio which affect the complexation and extraction of TFPH-Pd(II), have been investigated. Under optimized conditions, linearity was observed in the range of 0.5-17 μg mL-1with detection limit (S/N) of 0.038 μg mL-1, precision in range of 1.18-1.92%, accuracy as the %Erelof 2.4% and recoveries ranged from 101.7 to 104% with mean value of 102.4±0.135. The proposed method was applied for the determination of TFPH in the drug stelazine by both direct calibration and standard additions procedures and found to be 4.88 and 4.87 mg per unite, respectively compared with the stated value of 5 mg per unite. This method is also compared statistically with direct determination by using UV-Vis spectrophotometric technique which is preformed in our laboratory and found to be insignificant at 95% confidence level. All statistical calculations were implemented via the chemsoftware Minitab version 11.

Highlights

Trifluoperazine hydrochloride (TFPH),chemically 10-[3-(4-methylpiperazin-1-yl)propyl]-2(trifluoromethyl)phenothiazine hydrochloride (Figure 1), is a derivative of phenothiazine, S434 Z
UV-Vis spectra of the pure TFPH drug and its complex with Pd(II) were recorded using Shimadzu model UV-160 equipped with 10 mm matched quartz cell for recoding the spectra at 50 mg L-1 of TFPH standard solution and TFPH-Pd(II) in benzyl alcohol to verify of the formation of complex
An alternative indirect atomic absorption spectrometric (AAS) method has been reported for the analysis of trifluoperazine hydrochloride in pure form and pharmaceuticals

Summary

Introduction

Trifluoperazine hydrochloride (TFPH),chemically 10-[3-(4-methylpiperazin-1-yl)propyl]-2(trifluoromethyl)phenothiazine hydrochloride (Figure 1), is a derivative of phenothiazine, S434 Z. An Indirect Atomic Absorption Spectrometric Determination S439 correlation coefficient .The regression calibration equation obtained was; A=8.6x10-3 C +3.81x10-3 (where A is the absorbance and C the TFPH concentration as μg L-1) with a correlation coefficient of r= 0.9989 and the coefficient of determination (R2) of 99.78% which suggests statistically valid.

Results

Conclusion