Abstract
Liver cells (HepG2) were treated with well known toxic aromatic hydrocarbons 1-Naphthol and Dibenz[a,h]anthracene. Naphthol at 20-40µg/ml affected significant increase in the levels of reactive oxygen species in the cells.. Reactive nitrogen intermediates increased to an extent of 2.4 to 3.8 fold in cells treated with naphthol over a range of 20-80 µg/ml. Expression of antioxidant enzyme Glutathione peroxidase was found to increase two fold, while catalase expression was found to decrease by 21% on exposure to 1-Naphthol. Dibenz[a,h]anthracene did not affect discernible changes in the expression of these enzymes Induction of CYP1A1 mRNA by Naphthol at 60µg/ml was around 45 fold higher than untreated cells. Dibenz anthracene at a dose of 5µg/ml was found to be a potent inducer of CYP1A1 as it elevated expression of CYP1A1 mRNA to an extent of 350 fold. . Naphthol exhibited lipogenic potential in HepG2 cells. Concommitant increase in Sterol regulatory element binding protein 1c was observed.
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