Abstract
The aim of this work was to investigate the total phenolic content and antioxidant potential of aqueous extract of Phyllanthus amarus. The aqueous extract of Phyllanthus amarus whole plant was prepared then systematically investigated by various antioxidant assays, including: DPPH- (1, 1-diphenyl-2-picrylhydrazyl radical), Nitric oxide radical inhibition activity, and β–carotene-linoleate method, compared with ascorbic acid and rutin. Its total phenolic content was also measured by Folin-Ciocalteu colorimetric method. P. amarus aqueous extract exhibited effective antioxidant activity in dose-dependent manners and its IC50 values were calculated as 4.21±0.379, 426±0.512, 126±0.348 for DPPH, Nitric oxide radical inhibition activity, and β–carotene-linoleate assay respectively. Its total phenolic content was 52.71± 0.214 mg GAE /g. P. amarus aqueous extract has effective in vitro antioxidant activity which may attribute to its total phenolics, among which phyllanthin and hypophyllanthin can be considered as the active components. The pharmacological effects or healthcare functions of P. amarus may result from the synergistic effects caused by the combination of its components and its antioxidant effect plays an important role in the synergistic effects.
Highlights
Reactive oxygen species (ROS) such as superoxide radical anion, hydroperoxyl radical are generated in cells from byproducts of metabolism and responsible for the development of a wide number of degenerative diseases such as cardiovascular disease (CVD), diabetic, cirrhosis and several cancers[1]
All organisms have their own antioxidant defense system to protect them against free radical damage by enzymes, such as superoxide dismutase and catalase or compounds such as ascorbic acid, tocopherol and glutathione 2
The Total phenolic content (TPC) results were expressed as mg gallic acid equivalent as this compound represents the most simple form of a phenolic compound
Summary
Reactive oxygen species (ROS) such as superoxide radical anion, hydroperoxyl radical are generated in cells from byproducts of metabolism and responsible for the development of a wide number of degenerative diseases such as cardiovascular disease (CVD), diabetic, cirrhosis and several cancers[1]. Phytochemicals are produced by the plant kingdom for defense, protection, cell to cell signaling and as attractants for pollinators7are bioactive non-nutrient plant compounds that have been linked to the reduction of risk related to chronic diseases Their commercial potential can be utilized in food industry as additives, as antibiotics in pharmaceutical and as essence extract in herbal and cosmetic products. India is a land of biodiversity; this biodiversity supports numerous species of medicinal plants One of such genus that have been used for in folk medicine for decades and known to possess great diversity of secondary metabolites is the genus Phyllanthus (Euphorbiaceae). This study was undertaken to determine the total phenol content and antioxidant potential of aqueous extract of P. amarus
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