Abstract
As the current cancer treatment success rate is not sufficient, interest has grown in plants as possible sources of anti-cancer compounds. One such plant with a broad spectrum of activity is Lenourus sibiricus of the family Lamiaceae. This study investigates for the first time both the genotoxic and cytotoxic activities of TR (transformed) and AtPAP1 TR (with over-expression of transcriptional factor) root extracts of Lenourus sibiricus against various cancer cell lines (CCRF-CEM, K-562 and A549). Both tested extracts showed a cytotoxic effect on CCRF-CEM and K-562 cell lines, but strongest activity was observed for the AtPAP1 TR extract. No cytotoxic effect was observed against the A549 cell line in the tested concentration range, and it was found that both tested extracts may induce apoptosis by decreasing mitochondrial membrane potential and inducing nDNA damage lesion in the TP53 region and mtDNA in ND1 (mitochondrially encoded NADH: ubiquinone oxidoreductase core subunit 1) and ND5 (mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 5) regions in K-562 and CCRF-CEM. Our results confirmed that TR and AtPAP1 TR root extracts from L. sibiricus are cytotoxic and genotoxic against different model cell lines (CCRF-CEM and K-562). However, the observed genotoxicity of both extracts needs to be confirmed by additional studies. These preclinical observations support the use of L. sibiricus with other pharmacological purposes.
Highlights
Cancer remains one of the leading causes of morbidity and mortality globally, following cardiovascular disease [1]
In this experiment we investigated the cytotoxic activity of the transformed roots (TR) and AtPAP1 TR root extracts of
In this experiment we investigated the cytotoxic activity of the TR and AtPAP1 TR root extracts a
Summary
Cancer remains one of the leading causes of morbidity and mortality globally, following cardiovascular disease [1]. The natural biologically-active compounds they contain, have played a significant role in drug discovery and offer potential for development as therapeutic agents [4]; of approximately more than 300,000 plant species reported, only a small percentage has been the subject of phytochemical and biological activity studies [5]. A great part of the pharmaceuticals available are still derived from. In the past few years, considerable advancements have been made in the natural products endowed with antimutagenic and anticarcinogenic properties. Natural compounds known as dietary chemopreventive products offer a great potential in the fight against cancer through various mechanisms, including antioxidant activity, antimutagenic activity, enzyme modulation, gene expression and apoptosis, which have been tested in various cellular models [9]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
