Abstract
The combination of an expiratory CO 2 flow system and a scintillation counting system is described for the continuous counting of respiratory 14CO 2 exhaled from a mouse. Respiratory H 2O is trapped by a cooling trap to eliminate quenching effects of water on the counting. Dried air with expiratory 14CO 2-gas is fed to a given volume of the hyamine scintillation fluid in the reservoir and is bubbled in the fluid to trap 14CO 2-gas. A portion of the radioactive scintillation fluid is fed into a scintillation probe and returned to the reservoir continuously by connecting the reservoir and the probe with fine teflon tube. In the probe, the tube is coiled and placed between opposing photomultiplier tubes. The probe is connected with 2 ratemeters following a recorder for continuous counting of radioactivity in the flow stream. This device is particularly suited for the continuous counting of expiratory 14CO 2 with high counting efficiency as well as that of a liquid scintillation counter. The expiratory 14CO 2 patterns did not vary with the date, if the same mouse was used during 10.00 a.m. – 1.00 p.m. The patterns of different mice also were almost concordant. Discussions are described in detail for the comparison of respiratory 14CO 2 patterns given by this device and the other counters.
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