An Improved Micropropagation Protocol by Ex Vitro Rooting of Passiflora edulis Sims. f. flavicarpa Deg. through Nodal Segment Culture.

Mahipal S. Shekhawat,C. P. Ravindran,M. Manokari

doi:10.1155/2015/578676

Abstract

A procedure for rapid clonal propagation of Passiflora edulis Sims. f. flavicarpa Deg. (Passifloraceae) has been developed in this study. Nodal explants were sterilized with 0.1% HgCl2 and inoculated on Murashige and Skoog (MS) basal medium. The addition of 2.0 mgL−1 6-benzylaminopurine (BAP) to MS medium caused an extensive proliferation of multiple shoots (8.21 ± 1.13) primordial from the nodal meristems. Subculturing of these multiple shoots on the MS medium augmented with 1.0 mgL−1 of each BAP and Kinetin (Kin) was successful for the multiplication of the shoots in vitro with maximum numbers of shoots (25.73 ± 0.06) within four weeks of incubation. Shoots were rooted best (7.13 ± 0.56 roots/shoots) on half strength MS medium supplemented with 2.0 mgL−1 indole-3 butyric acid (IBA). All in vitro regenerated shoots were rooted by ex vitro method, and this has achieved 6-7 roots per shoot by pulsing of cut ends of the shoots using 200 as well as 300 mgL−1 IBA. The plantlets were hardened in the greenhouse for 4-5 weeks. The hardened plantlets were shifted to manure containing nursery polybags after five weeks and then transferred to a sand bed for another four weeks for acclimatization before field planting with 88% survival rate.

Highlights

Passiflora edulis Sims f. flavicarpa Deg. is an important species of the family Passifloraceae, distributed mainly in the tropical and the subtropical regions of the world
It is native to Brazil and the fruits are mainly used for processing of juice
In Brazil, it is cultivated at commercial scale and the fruits are consumed as juices and in ice cream making [2]

Summary

Introduction

Passiflora edulis Sims f. flavicarpa Deg. (passion fruit) is an important species of the family Passifloraceae, distributed mainly in the tropical and the subtropical regions of the world. MS medium augmented with BAP and Kin ranging from 1.0 to 5.0 mgL−1 was used for the initiation of the shoots from nodal meristem of the explants. The MS medium supplemented with cytokinins (BAP and Kin) ranging from 0.5 to 2.5 mgL−1 was used for multiplication of shoots.

Results

Conclusion