Abstract
Prion diseases are characterized by the accumulation of a pathological form of prion protein (PrP Sc), which behaves as an infectious agent. Here we developed an in vitro co-culture system to analyze the PrP Sc transmission from ScN2a cell, which persistently retains PrP Sc, to naïve N2a cell. In this cell-to-cell transmission system, PrP Sc transmitted to recipient N2a cell was able to be detected within 5–7 days. Further characterization showed that higher cell density greatly facilitated the transmission of PrP Sc. This improved in vitro transmission method may become a useful tool for unveiling the molecular mechanism of PrP Sc transmission.
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Schedule a callMore From: Biochemical and Biophysical Research Communications
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