Abstract
Among the organochlorine pesticides, DDT and HCH are the major cause for food and environmental contamination. To detect them effectively in water samples an immobilized dehydrohalogenase based potentiometric biosensor was developed. The chloride ion released as a result of dehalogenation by immobilized dehydrohalogenase during the degradation of DDT and HCH, respectively, was detected using an ion selective electrode. The voltage response had a direct linear relationship with the concentration of chloride (Cl−) released by DDT and HCH, respectively. The immobilization was advantageous in improving the enzyme property and could be used repeatedly without losing activity.
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