Abstract
Objective To evaluate the effect of periprosthetic high glucose environment on the growth of Staphylococcus and bacterial biofilm formation. Methods New Zealand white rabbits were used to establish the animal model of prosthetic infection. There were staphylococcus aureus group (SA, n=40) and Staphylococcus epidermidis group (SE, n=60). Each group were randomly divided into the experimental group and control group. The bacteria were injected with 0.1ml l%glucose into the knee of rabbits in experi-mental group; the rabbits were injected with 0.1ml normal saline in the control group. The SA group were observed in the 2,4,6 and 8 days; The SE group were observed at 2,4,6,8,12 and 16 days. Bacterial counts, scanning electron microscopy and histological observations were used to the changes. Results In the SA group, there was no difference in bacteria counts of synovial fluid between the experimental group and control group 2 days after injection (P=0.426). But 4,6 and 8 days after injection ,the bacteria counts of synovial fluid in the experimental group increased significantly. Scanning electron microscopy found On the day of 2,4,6, the number of bacteria adhere to the prosthetic surface had increased significantly in experi-mental group, compared with the control group.The bacterial biofilm formed significantly. Histological exami-nation revealed that the bacteria were found in the tissue surrounding prosthesis two days after injection,but not in the control group. In SE Group no difference was found in bacterial counts of synovial fluid between the experimental group and control group after 2,4,6,8,12 and 16 days after injection,as well as the num-ber of bacteria adhere to the prosthetic surface Histological examination revealed that no bacteria was found in the tissues around prosthesis in two groups. Conclusion High glucose environment has significant effect on prosthetic infection by Staphylococcus aureus, but not those caused by Staphylococcus epidermidis. Key words: Knee prosthesis; Infection; Staphylococcus aureus; Staphylococcus epidermidis; Glucose
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