Abstract
Objective To investigate the feasibility of mimetically osteoinduetive bone in promo-ting spinal fusion of rabbits.Methods With aid of biological gels compound and dynamic (oscillating pattern) seeding,three-dimensional construction of mimetically osteoinductive bone was made by implan-ting adipose derived stromal cells (ADSCs) into rhBMP-2 mediated Cs-Col-β-TCP matix.Lumbar 4-5 in-tertranverse process fusion was established in New Zealand rabbits.In group A1 the rabbits were transplan-ted by mimetically osteoinduetive bone,those in group A2 by bone autograft,those in group BI by rhBMP-2 mediated Cs-CoI-β-TCP matix, and those in group B2 by Cs-Col-β-TCP matix as blank scaffold.Histolo-gy, immunohistochemistry, new bone ratio, BMP quantification, and palpation and biomechanieal examina-tions were performed at different time points.Results Compared with other groups, group A1 displayed remarkable fusion capacity with statistical significance according to the data of new bone ratio, BMP con-tent and biomeehanical tests (P<0.05).Conclusion Enhanced osteogenic potential can be achieved by using mimetically osteoinductive bone, and spinal fusion can be promoted with concord pattern of osteoin-duetive and osteoconductive effects. Key words: Spinal fusion; Mimetically osteoinductive bone; Adipose derived stromal cells; Chitosan; Collagen
Published Version
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